Applications for PacBio circular consensus sequencing

I am a fan of the Pacific Biosciences PacBio RS sequencing instrument, as some of the readers of this blog maybe already are aware of. We use data from this instrument in our work, and the Norwegian Sequencing Centre – with which I am affiliated – offers the technology to its users.

PacBio recently increased their read lengths. With this so-called C2XL chemistry, average raw length is now around 4.3-4.5 kbp and maximum read lengths can go over 20kbp. These extra long reads are great for de novo genome sequencing applications, something we are trying ourselves. However, a bit buried in the news about these longer reads is the consequences for PacBio’s so-called Circular Consensus Sequencing, or CCS.

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