As before, full run throughput in gigabases (billion bases) is plotted against single-end read length for the different sequencing platforms, both on a log scale:
Earlier this year, I started a petition to ask Roche/454 Life Sciences to make the Newbler software (gsAssembly, gsMapper and Amplicon Variant Analyzer) open source. See this post for the background to the petition.
When I closed the petition, 162 people had signed it, see the PDF on figshare. During the Advances in Genome Biology and Technology (AGBT) meeting in Florida, I handed over the results of the petition to two Roche representatives, Dan Zabrowski, Head of Roche Sequencing Unit and Paul Schaffer, Vice President of Roche 454 Sequencing Business, see my blog post on the conversation I had with them.
Dan Zabrowski and Paul Schaffer promised me an official Roche response, and here it is (exclusively released through this blog): Continue reading
A couple of weeks ago, I started a petition to ask Roche/454 Life Sciences to make the Newbler software (gsAssembly, gsMapper and Amplicon Variant Analyzer) open source. See this post for the background to the petition.
The results are in, see the PDF on figshare. 162 people have signed the petition. Many thanks to all of you!
This week, I attended the Advances in Genome Biology and Technology (AGBT) meeting in Florida, and on Thursday I handed over the results of the petition to two Roche representatives, Dan Zabrowski, Head of Roche Sequencing Unit and Paul Schaffer, Vice President of Roche 454 Sequencing Business.
I had a half hour, very open and interesting discussion with Roche. Roche expressed their appreciation of the fact that we as a community voiced our concerns and wishes around Newbler. Roche occasionally picks up signals from researchers, but a petition like this was very useful for them as a much stronger signal of what we think about one of their products.
Dan Zabrowski told me Roche is committed to fully support access to the Newbler software even after the 454 Life Sciences shutdown. They will take the request for open source access to the code seriously, and promised to come with an official response somewhere in the coming weeks. They did not hint at what that response would be, which is understandable.
I want to thank Dan Zabrowski and Paul Schaffer for giving me time to explain the background and hand them the results. I also again want to thank all of you who signed the petition. We may collectively have made a difference. Keep an eye out on my twitter feed and this blog for the official Roche response!
The one and only Oxford Nanopore talk at AGBT 2014 – with real data
Twitter started buzzing this morning at AGBT because researchers started getting confirmation emails from Oxford Nanopore regarding their application for the MinION Access Program (MAP). This timed well with the first talk discussing some serious data from the platform, by David Jaffe from the Broad Institute, entitled “Assembly of Bacterial Genomes Using Long Nanopore Reads”. Probably no coincidence…
David Jaffe’s highly anticipated talk showed data generated by Oxford Nanopore on their MinION from two bacterial genomes. One was a methylation negative E coli (the fact that it was methylation negative may have been significant, but he didn’t say). The second species was a Scardovia. 5 micrograms of DNA were sent to the company. Library prep consisted of fragmentation and adaptor ligation, basically the classical workflow. Nothing was said about the type of adaptors.
My petition to ask Roche/454 Life Science to make the Newbler software open source, as announced on this blog, has so far yielded 124 signatures. Thanks to all who signed!
It is not to late to add your signature!
Please do this before Thursday February 13th. On that day, at the Advances in Genome Biology and Technology (AGBT) meeting, I have been given the opportunity to hand over the petition results to two Roche representatives: Dan Zabrowski, Head of Roche Sequencing Unit and Paul Schaffer, Vice President of Roche 454 Sequencing Business. The fact that Roche opens up for me meeting these people makes me very happy!
Finally, here are some of the comments added by people who signed the petition. I anonymised them, but if you see your comment and would rather not I repeat it here, let me know (lex dot nederbragt at ibv dot uio dot no). Thanks for these wonderful comments on the Newbler assembly:
Roche has an excellent opportunity of publicit their commitment with science making their fantastic product Newbler open-source
I would like to runProject forever!
Newbler source code is very valuable to the sequencing community. Roche will be doing a service to the scientific community by making the Newbler source code available after the 454 shutdown.
Goodwill from making this OS is best value Roche can get for this code
This is way overdue. James Knight has done wonderful work but Newbler may fall through the cracks if it is not opened up to the community.
The newbler is great assembler for paired end data.
Please make it opensource, and I would be happy to contribute to it’s development.
Open source is a friend to science aiding reproducibility and allowing future developers to learn from existing work. Please do not limit science by preventing our access.
The Newbler suite has been remarkably useful and should continue to be so. I hope Jim Knight sees this and pushes for his creation to be made open source
I strongly support this petition and note there is a precedent for a commercially-developed assembler to be made publicly available under an open source license – the Celera assembler – much to the benefit of the scientific community.
Newbler was the basis for contig assembly of MiSeq data for me. An excellent piece of software.
Newbler was always my favorite thing about 454. It deserves to live on.
(Cross-posted at contig.wordpress.com)
The Newbler assembler and mapper (gsAssembler, gsMapper) was developed especially for working with the reads from the Roche/454 Life Science sequencing technology. It is one of the best programs to deal with this type of data, scoring well in the assemblathon 2 competition. Newbler has been used for many large and small genome assemblies (numerous bacteria, Atlantic cod, bonobo, tomato, to name a few). Recently, Newbler has added support for using multiple sequencing technologies, making it one of the few hybrid assembly programs available. At the Advances in Genome Biology and Technology (AGBT) in 2013, Roche announced having used the Newbler program with a hybrid 454 and Illumina dataset to improve upon the human genome.
However, the Newbler program is not open source. Luckily, researchers only need to fill out an online form to get a free copy of the software. Still, this has hampered the wide-spread adoption of this program. Newbler, for example, was not included in assembly evaluations like GAGE and GAGE-B. That Roche/454 does not want to make the source code for Newbler available is partly understandable from a commercial standpoint: at least one competitor technology (Life Tech/Ion Torrent) with a similar sequencing error-model could benefit from access to the code. In fact, in a blog post, I showed Newbler to be superior to an open-source program when assembling Ion Torrent mate-pair data.
More worringly is that the hundreds of projects that used Newbler as part of the analysis are fundamentally irreproducible without the source code for each of the different versions. This is especially the case for projects, such as the Atlantic cod genome project, that have been given access to development versions of the code, incorporating elements not available to the general community.
Last October, Roche announced it will shutdown its 454 sequencing business in mid-2016. Whatever one may feel about this decision, this further strengthens the argument for Roche/454 to make the Newbler source code open source. After the 454 shutdown, Newbler is otherwise likely to disappear too, meaning that large swathes of the literature cannot be recapitulated from the raw data. Also, long after the 454 shutdown, many researchers will have to process their 454 sequencing data, and many may still want to rely on Newbler for that purpose.
There are several other reasons why I feel the research community should be given access to the source code of Newbler. Newbler represents a very valuable contribution to the field of genome assembly and mapping. Software developers can learn from the algorithms and implementations of the Newbler code, opening up for reusing these in other programs. Also, there is the hope that developers will improve upon the program, for example by adding support for other sequencing technologies, or assembling with reads longer than the current maximum of 2 kbp.
So I hereby ask the readers of this blog for help: I have set up an online petition asking for Roche/454 to make the Newbler source code available at the latest at the time of the 454 shutdown. Please sign the petition here. Additionally, spread the word (e.g., on twitter or your own blog). Thanks in advance!
I intend to hand over the results of the petition to a Roche representative at the Advances in Genome Biology and Technology (AGBT) meeting (February 12-15, 2014).
Finally, feel free to use the comments to tell me about your Newbler experiences!
(Thanks to Nick Loman for his constructive comments on an earlier version of this post)
On the Oxford nanopore MinIon Access ProgramLet’s say you hear about this company that claims to have a revolutionizing clothes washer, and you get to try it out! For a refundable $1000, they will ship you a washer, and they have plenty of their special washing powder available, which they will happily sell to you. The thing is, though, the company hasn’t shown anyone how good it actually works, how fast it works, and how clean your clothes will become. Not just that, once you have the washer, you’ll need to first use it on dirty clothers they will send you, and you’ll have to send them back the results. Also:
Only after achieving “consistent and satisfactory performance” with the test samples will participants be allowed to run their own [clothes]
Would you buy that washing machine? I guess not.
In December last year, I posted a visualisation of the developments in high-throughput sequencing on this blog. In this field the technologies change rapidly, so it is about time for an update. Here is the October 2013 installment. Full run throughput in gigabases (billion bases) is plotted against single-end read length, both on a log scale:
With this post I present a figure I’ve been working on for a while now. With it, I try to summarise the developments in (next generation) sequencing, or at least a few aspects of it. I’ve been digging around the internet to find the throughput metrics for the different platforms since their first instrument version came out. I’ve summarised my findings in the table at the end of this post. Then, I visualised the results by plotting throughput in raw bases versus read length in the graph below.
A potential user (‘customer’) of our sequencing platform asked how to generate reference genomes for his 4 bacterial strains. His question inspired me to write this post. The suggestions below are not absolute, just my thoughts on how one these days could go about sequencing a bacterial genome using one or more of the sequencing platforms. I would appreciate any feedback/suggestions in the comments section!
Option 1: bits and pieces